In vitro complementation between nonallelic Drosophila mutants deficient in xanthine dehydrogenase.
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چکیده
منابع مشابه
A Third Locus (lxd) Affecting Xanthine Dehydrogenase in Drosophila Melanogaster.
WO genes which affect xanthine dehydrogenase in Drosophila melanogaster Tare already known. These are maroon-like (ma-1) on the X chromosome, and rosy ( r y ) on the third chromosome. The phenotype of both of these mutants is a dark red-brown eye color. When either of these genes is homozygous, the mutant flies have no detectable amounts of xanthine dehydrogenase (FORREST, GLASSMAN, and MITCHEL...
متن کاملElectrophoretic variants of xanthine dehydrogenase in Drosophila melanogaster.
HE activity of xanthine dehydrogenase (XDH) in Drosophila melanogaster is affected by mutations at three loci: ma4 at locus 64ion the X chromosome, ry at locus 52 ion the third chromosome, and lxd at locus 33 ? on the third chromosome. See GLASSMAN (1965) for a discussion of these loci. The ma4 and the ry mutants are deficient in XDH while lzd flies have 20 to 25% of the XDH activity of wild ty...
متن کاملGenetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster.
Experiments are described that provide an opportunity to estimate the genetic limits of the structural (amino acid coding) portion of the rosy locus (3:52.0) in Drosophila melanogaster, which controls the enzyme, xanthine dehydrogenase (XDH). This is accomplished by mapping experiments which localize sites responsible for electrophoretic variation in the enzyme on the known genetic map of null-...
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We searched for novel Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting sequence (PTS) of Pex3p (amino acid residues 1–40)-fused enhanced green fluorescent protein (EGFP). From mutagenized TKaEG3(1–40) cells, the wild-type CHO-K1 stably expressing rat Pex2p and of rat Pex3p(1–40)-EGFP, numerous cell colonies resistant to...
متن کاملIn vitro assembly of Neurospora assimilatory nitrate reductase from protein subunits of a Neurospora mutant and the xanthine oxidizing or aldehyde oxidase systems of higher animals.
In vitro assembly or complementation of a hybrid assimilatory nitrate reductase was attained by mixing a preparation of nitrate-induced N. crassa mutant nit-1 specifically with acid-treated (pH 2.5) bovine milk or intestinal xanthine oxidase, rabbit liver aldehyde oxidase, or chicken liver xanthine dehydrogenase. The complementation reaction specifically required induced nit-1, the only nitrate...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 48 شماره
صفحات -
تاریخ انتشار 1962